This video tutorial is an easy step-by-step guide for using the NCBI BLAST bioinformatics tool for your genomic research. We walk you through how to do a nucleotide or protein BLAST and give you tips for interpreting your BLAST results. We will also show you how to create an evolutionary tree from your BLAST results and demonstrate how to use BLAST for multiple sequence alignment. With the information provided in this tutorial, you should be ready to tackle your own bioinformatics and genomic research questions in no time!