This video tutorial provides a quick overview of the NCBI website. We walk you through how to search for nucleotide and protein sequences using NCBI’s databases and explain what information is provided with each sequence. We also give you tips on how to interpret gene data and guide you through the process of assessing gene variants and mutations using NCBI’s Variation Viewer. With the information provided in this video, you should be well equipped to start your own bioinformatics or genomics adventure!